How to deal with human DNA contamination of your DNA sequencing: an example from a Malawian dance garment. » blocking primer

Hypothetical alignment of human and another mammal’s DNA sequence (reference DNA sequences). DNA primers have been designed to these sequences. The standard primer matches both mammal DNA sequences exactly. The blocking primer matches human DNA (bases that match human sequence but not the other mammal sequence are shown in red). The blocking primer ends with a C3 spacer, which stops copies being made of the human DNA.Read more

Hypothetical alignment of human and another mammal’s DNA sequence (reference DNA sequences). DNA primers have been designed to these sequences. The standard primer matches both mammal DNA sequences exactly. The blocking primer matches human DNA (bases that match human sequence but not the other mammal sequence are shown in red). The blocking primer ends with a C3 spacer, which stops copies being made of the human DNA.