Colossal squid


The formalin has been pumped out of the tank and the squid will be rinsed in fresh water before the lift on Wednesday.

Preparing the pump

Preparing the pump - 3170 - © Copyright Museum of New Zealand Te Papa Tongarewa, 2008

Mark Fenwick checking the squid as the formalin level drops

Mark Fenwick checking the squid as the formalin level drops - 3171 - © Copyright Museum of New Zealand Te Papa Tongarewa, 2008

The squid emerges

The squid emerges - 3172 - © Copyright Museum of New Zealand Te Papa Tongarewa, 2008

The specimen looks to be in good condition (considering!). The sucker rings and hooks are intact, so the buffering of the formalin solution worked well. The rip in the mantle is larger than we thought, so turning it over on Wednesday is going to be a tricky task!

Tomorrow we will be setting up the webcams…

Specimens in museum collections are usually preserved in a 70 per cent aqueous solution of ethyl alcohol or in a 2-4 per cent solution of formaldehyde (5-10 per cent formalin).

The great disadvantages of ethyl alcohol are that it is flammable, it volatilizes very readily, it tends to produce precipitates from sea-water, and it causes shrinkage of many specimens. Although formalin is not flammable, and it evaporates less rapidly than ethyl alcohol, precautions to prevent desiccation are still necessary; and it has the further disadvantage that it tends to oxidize to formic acid, which may dissolve calcareous structures, and it has a very strong smell and is toxic.

Glycol experiment - squid

Glycol experiment - squid - 3169 - © Copyright Museum of New Zealand Te Papa Tongarewa, 2008

Both ethanol and formalin cannot be used in large volumes in public galleries because of the hazardous nature of the chemicals. So in order to display our colossal squid we will be using a glycol solution with an antimicrobial preservative to prevent microbial growth. We have been experimenting with smaller specimens in jars. Initial results indicate the system will work quite well – although there has been some discoloration, replacing the mix with a second fresh glycol solution does not result in more leaching of the colour, so our plan to replace the glycol before the specimen goes on display should work! Specimens have been preserved for months or years in glycol, but nobody has tried preserving such a large (495 kg) specimen in glycol before, so this is quite a learning curve for us!

The glycol will be diluted to between 20-30% with water. To prevent the growth of microorganisms (which could happen as the mixture contains sufficient water to support microbes) we will add a small quantity of Dowanol preservative. This will allow us to have 5,000 litres of liquid in the public galleries, while meeting safety and health requirements.

Another concern is that if the soultion becomes acidic the hooks and sucker rings will be dissolved – to prevent this happening we will ‘buffer’ the solution with limestone chips to keep the pH neutral. Although glycol has been used successfully for long term storage of specimens on a small scale, the use of large quantities for the colossal squid is in many ways experimental, and one reason why we will be monitoring the specimen constantly while it is on display.

Squidcam’s back!

We’re moving the colossal squid out of formalin and into its new display tank. Webcam viewing will be available live on Wednesday 6 August starting 9am NZ time (USA: Tuesday 2pm to 5pm, UK: Tuesday 10pm), for one day only.

Check out the full programme on our website: http://www.tepapa.govt.nz/squid/

Mark Fenwick with the lifting cradle

Mark Fenwick with the lifting cradle - 3168 - © Copyright Museum of New Zealand Te Papa Tongarewa, 2008

Preparations are underway for lifting the specimen out of the fixing tank. Te Papa staff Gary Dawson and Steven McStay built us a lifting frame to fit one of the whale lifting mats we have. Once we have drained the formalin, the tank will be refilled with water to rinse the specimen and allow us to position the squid on the mat. The fixing process will have made the tissues much firmer so we will be able to turn the specimen over – something we could not do before without the risk of seriously damaging it!

Construction of the new colossal squid display tank is almost complete, and we hope to transfer the squid into it on Wednesday 6th August.

Planning the lifting

Planning the lifting - 3167 - © Copyright Museum of New Zealand Te Papa Tongarewa, 2008

The design of a tank to safely store and display the specimen has presented Te Papa with a considerable challenge and we have been assisted by Wellman Associates Ltd in Hawera and Etech Industries in Palmerston North.

Tank dimensions are approximately 4.6m long x 1.6m wide x 0.9m deep, and the design needed to make allowance for:

  • lighting inside the tank;
  • dealing with possible air bubbles in the storage fluid;
  • it had to allow access for sampling the storage fluid to check acidity levels and for any signs of bacterial contamination;
  • there has to be flexible panels to allow for contraction and expansion of the volume of fluid with temperature changes;
  • Plus, allowing easy access for us to lift the 495 kg specimen in and out!

All of which has to be achieved while still making it easy for visitors to see the squid – including providing wheelchair access.

proposed tank design from Caldwell Engineers

proposed tank design from Caldwell Engineers - © Copyright Museum of New Zealand Te Papa Tongarewa, 2008

The specimen will be displayed with around 5,000 litres of storage solution. The total weight of the specimen, fluid and tank has meant we have had to check the floor loadings to find a suitable location for the display! Before we began, we looked at existing public displays of giant squid specimens around the world and considered two different tank types – stainless steel tanks with an acrylic lid which allow visitors to look down at the squid, but offers limited viewing from the side, or clear acrylic tanks which allow visitors to view the whole squid side on.

Finally we made the decision to build a stainless steel tank with an acrylic lid because this allows us to use more dramatic lighting without problems of reflection (an acrylic tank is much more difficult to light because of this). Any clouding of the fluid, or accumulation of debris from the specimen will be less obvious as it will sink to the bottom, and a curved acrylic lid will allow air bubbles to float to the sides and not impede the view of the squid. The mounting system for the specimen (a series of Perspex rods to hold the specimen in place) will be less obvious, and the top down view into the stainless steel tank will give more of a sense of “mystery and drama”.

The specimen has had several weeks in the formalin fixing solution, and is now much more rigid, so we will be able to handle it much easier than before when there was a risk of tearing the soft tissues. Firstly we will turn the specimen over (we still haven’t seen the top side!) and repair a split in the mantle which was damaged during the process of landing the squid on the fishing boat. Once the repairs have been made and the squid manoeuvered onto a lifting mat, we will lift it out of the fixing tank and into the display tank. We hope to have the webcam operational again, so viewers will be able to watch the process – details to come as soon as we finalise dates and times!

Proposed location of the colossal squid display in "Mountains to Sea" gallery.

proposed location of the colossal squid display in the gallery - © Copyright Museum of New Zealand Te Papa Tongarewa, 2008

The transfer of the specimen to the display tank is only the first step. Once moved to the new tank the squid will have to be mounted on acrylic supports so that it doesn’t collapse into a humongous blob at the bottom, and rotated slightly so the eye can be seen. As the beak is concealed at the centre of the arms, we will also be displaying the beak (and various other parts, such as eggs, suckers and hooks), from the smaller dissected colossal squid – all of this part of the exhibition will have to be constructed, and text and labels prepared before we can even start thinking about installation in the gallery later in the year. Construction of the tank was contracted out to E-tech Industries in Palmerston North, while most of the display will be prepared in-house at Te Papa.

On Thursday 17th of July, Melbourne Museum (Victoria, Australia), held its first ever public dissection by museum scientists of a giant squid.  

The dissection  was performed in the Science and Life Gallery and the dissection was streamed live. A recording can be viewed here

 http://museumvictoria.com.au/About/MV-News/2008/Giant-squid-public-dissection-at-Melbourne-Museum/

The squid which was accidentally caught in a fishing trawl off western Victoria, weighs around 245 kg, making it the largest giant squid that Australian researchers have encountered. It is estimated that, intact, the animal would have been over 12 metres long.

The hooks seem to grab everyone’s attention, so here’s an update

tentacle club swivelling hook and arm sucker

tentacle club swivelling hook and arm sucker dissected out from the fleshy suckers

Other squid families have hooks on the arms, or the tentacles, or both, but the colossal squid is the only hooked squid in its family (the Cranchiidae, about 20 species). It possesses hooks on each of the eight arms, and also on the club ends of the two long tentacles, but the arm-hooks and tentacle-hooks are very different.

The tentacular hooks are the swivelling hooks. Each sits on a short stalk, flush with the oral surface of the tentacle club, in a flattened depression that allows the flattened ‘back’ surface of the hook to rotate. The hooks can swivel 360 to 720 degrees, but it is not known whether the squid actively controls each hook individually, or whether the hooks swivel passively once latched onto the prey, in order to keep the best grip. There are 22 to 25 hooks in two rows on the middle part (manus) of the tentacle club, and each row is flanked by a row of tiny marginal suckers. The swivelling hooks are smaller than those on the arms and have only a singe main ‘claw.’

 

 

The arm hooks do not swivel. They are set in a double row in the middle of each arm, preceded and followed by the more standard toothed suckers. The arm hooks are set in fleshy, very muscular sheaths and are strongly attached to the arms. They are likely to assist in holding and immobilising struggling prey as it is being killed and eaten. Most of the arm hooks have the main strong ‘claw’, and also two smaller auxiliary cusps closer to the hook’s base, making them three-pointed and maximising their ability to hold and dig in. The base of each hook also has a complex structure that is set deep into the surrounding musculature.  The top picture shows the hook and serrated sucker im structure dissected out from the fleshy part of the suckers.

Non-swivelling arm hooks

Non-swivelling arm hooks

 

 

Getting the colossal squid onto display isn’t just a simple matter of building a tank and moving it into the gallery!

First of all we have to plan a few details – for example, because of the size of the tank and the amount of liquid it will contain, it will weigh over five tonnes, so the building engineers have to check that the floor loadings will cope.

Then we have to consider how to get the tank into the building once it is built – it may be too wide to fit through the door, so exhibition staff will have to decide if it can be lifted into the building through a window using a crane – fortunately the museum was designed with these issues in mind, so there are windows which can be removed relatively easily to do this (we’re hoping that  Wellington’s balmy weather won’t cause any delays).

Once the tank is in place we have to fill it with preservative. 5,000 litres of preservative will have to be mixed and pumped into the tank – that’s about 25x 200 litre drums which will have to be transported into the gallery and mixed to the correct concentration in the tank – something which will have to be done when the museum is closed to the public.

In the event of an earthquake there is the risk of the tank rupturing, so to prevent a tidal wave of preservative cascading through the collections, the entire floor of the gallery has to be bunded to contain any spillage – this will involve a complete rebuild of the gallery floor!  

As the gallery is a public space we cannot use the usual formalin or alcohol preservatives, but will be trialling a propylene-glycol mixture. (Glycol is used as anti-freeze and has been used previously to preserve specimens on a smaller scale, but we will need to design the tank so that we can take samples for testing on a regular basis to check the pH for acidity and any signs of deterioration of the specimen). 

Placement of the specimen inside the tank will require the construction of some supports to keep it in place – because of the different density of parts of the specimen, some parts, such as the mantle, will float upwards, while others, such as the arms, will sink.

While preparations are underway to get the tank built, designers are working on how to light the specimen so that it can be seen: we are going to use an LED system which will enable lights to be placed inside the tank to avoid problems of reflection from the liquid surface. Then there’s the supporting display with other specimens and photographs to be prepared and labels written, printed and installed.

Not to forget the squid itself! We are still planning on how to lift 495 kg of pickled  squid out of its temporary fixing tank, into the display tank and move it from the laboratory building (about 1 kilometre away) to Cable Street. The preservation process has made the tissues much stronger and less jelly-like, but so far we haven’t been able to even turn it over to see what condition it is in without the risk of tearing the mantle.

So far things are going to plan – we hope to have a date for the display before the end of the year.

How many colossal squid specimens are there in the world? And how many of these are on display? How good do they look?

The answer to the first question is probably four, and the answer to the next question, is none – at the moment. The display of Te Papa’s colossal squid specimen is going to be the first – as far as we know. But if you know of one that is already on display, please tell us!

‘How good do they look?’ This creates more questions – what sort of tank do we make to display our colossal squid in? How do we make it look good?

So we thought we’d have a look around the internet at other displays of big squid and get some help. We found quite a few giant squid – all in various states of display, some still looking glamorous, others not so good. If you’ve got a picture of your favourite big squid on display somewhere, please share it with us.

Meet ‘Archie’ (Architeuthis is the genus name for a giant squid), the 8.62 metre long giant squid on display in the Darwin Centre of the Natural History Museum in London. 
http://www.nhm.ac.uk/about-us/news/2006/feb/news_5255.html

At the Smithsonian Museum of Natural History in Washington DC, they’ve had a giant squid on display in a tank in their ‘Ocean Planet’ exhibit for qutie a few years. This specimen was washed ashore off Massachusetts in 1980. http://seawifs.gsfc.nasa.gov/OCEAN_PLANET/HTML/squid_Architeuthis.html

The search for a giant squid in New Zealand waters was also the subject of their exhibition Search for Giant Squid. http://www.mnh.si.edu/natural_partners/squid4/
It makes really interesting reading. Our New Zealand squid expert, Dr Steve O’Shea, was part of the expedition. It was Steve who lead the team at Te Papa when the colossal squid was examined in April.

Closer to home, there are giant squid on display in Australia. A New Zealand giant squid was put on show in a block of ice at the Melbourne Aquarium in 2005 and looked really impressive!
http://www.theage.com.au/news/national/new-squid-on-the-ice-block/2005/12/20/1135032018280.html

Here in New Zealand you can see a giant squid at Kelly Tarlton’s. Staff there nicknamed it ‘Squid Vicious’. http://www.nzherald.co.nz/section/1/story.cfm?c_id=1&objectid=10402608
Another, called ‘Maximum’, is at the National Aquarium of New Zealand in Napier.

We have to remember with our display tank that colossal squid are a different shape to giant squid – they have a much bigger, rounder body and the tentacles are shorter. Think fat and round, like a soccer ball, rather than the long cylinder shape and impressively long tentacles of its cousin, the giant squid. How would Trinnie and Susannah describe these body shapes, I wonder…

 

You can order this picture here

So if you’ve got a picture of your favourite big squid on display somewhere, or want to tell us about seeing one on display, please share it with us!

What’s happening with the colossal squid? Why haven’t we put it on display yet? Well the short answer is that good things take time – and money.

While the specimen is happily sitting in its storage tank, slowly preserving in the formalin, the squid team is planning for the next stage – how to put it on display at Te Papa.

There are a few things for us to consider – not least is how to build a tank to store it in that also lets visitors get a really good look at the specimen. We need quite a big tank to hold our 4.2 metre long colossal squid, weighing 495 kilograms.

And when you combine the weight of the squid, with the weight of the tank and the liquid, that’s quite a floor loading. So there are only a few places within the Te Papa building that we can put the tank and the squid in.

What are we going to tell you about the colossal squid? What would you like to see in the exhibition? These are all questions that we are now exploring as we develop the exhibition.

We’ll keep you posted through the blog on how we’re going, and give you a bit of an insight into the crazy world of exhibition development at Te Papa.

If you do have any questions for us, let us know!

Two of the lectures from the Te Papa squid lecture series are now available online:

Dr Steve O’Shea
Squid: Small, Giant, and Colossal 
http://www.r2.co.nz/20080520/steve.asx

Dr Eric Warrant and Dr Dan-Eric Nilsson:
The Weird and Wonderful Eyes of Animals Who Live in the Dark and the Deep Sea
http://www.r2.co.nz/20080520/eric.asx

 

 

We will unfortunately not be able to have other lectures online due to copyright issues.

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