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Supermarket Systematics at Te Papa’s Senior Science Careers Day

On June 25th, Te Papa hosted its annual Senior Science Careers Day. College students from several area schools came to get a glimpse of what it’s like to be a scientist working at Te Papa. Two of us from the Botany team participated in the event: Carlos Lehnebach (Curator) and Heidi Meudt (Research Scientist). We are both plant systematists, which means we name, describe and identify plant species and study their relationships with other species.
Curator of Botany Carlos Lehnebach introducing the activity to the Senior Science Careers Day students. Photo © Te Papa.

Curator of Botany Carlos Lehnebach introducing the activity to the Senior Science Careers Day students. Photo © Te Papa.

On Senior Science Careers Day, we thought it would be fun to let the students practice their systematic skills by taking part in a hands-on activity that we called “Supermarket Systematics”. 

We bought multiple sets of twelve fruits and vegetables from a local supermarket. 

The set-up for the Supermarket Systematics exercise. Could you classify these twelve fruits and vegetables into two main plant families? Photo © Te Papa.

The set-up for the Supermarket Systematics exercise. Could you classify these twelve fruits and vegetables into two main plant families? Photo © Te Papa.

The students’ task was to classify them into two plant families based on morphological characteristics.  Students worked together in groups and used dissecting microscopes to compare seeds, fruits, and leaves of the fruits and vegetables. 

The budding botanists from Hutt Valley High School classifying the fruits and vegetables. Photo © Te Papa.

The budding botanists from Hutt Valley High School classifying the fruits and vegetables. Photo © Te Papa.

They also had several photos that showed other parts of the plants such as flowers and habit, which provided further clues as to which plant family each fruit or vegetable belonged to. 

Some photos of additional information each group could use during the activity. Photo © Te Papa.

Some photos of additional information each group could use during the activity. Photo © Te Papa.

We spent time talking with the groups and asking them questions about their observations. 

Botany Research Scientist Heidi Meudt, together with Senior Education Programme Developer Emma Best, helping the Hutt Valley High students with a dissection. Photo © Te Papa.

Botany Research Scientist Heidi Meudt, together with Senior Education Programme Developer Emma Best, helping the Hutt Valley High students with a dissection. Photo © Te Papa.

Most groups did a great job as supermarket systematists! They were able to correctly classify ten of the items into two economically important plant families.

Brassicaceae (mustard family) Solanaceae (potato family)
cabbage potato
Brussels sprouts tomato
bok choy aubergine
broccolini capsicum
radish tamarillo

 

Some also correctly identified the two vegetables that did not belong to either family, which were kumara (sweet potato) and lettuce. Although kumara look very similar to potatoes, they are in a different family (Convolvulaceae) and the students confirmed this by looking at photos of other characteristics of each plant. In the same way, lettuce looks superficially similar to cabbage, but is in the daisy family (Asteraceae). 

We hope the students had as much fun as we did doing the Supermarket Systematics activity, and learned a bit about plant systematics and research at Te Papa too!

Getting a measure of plant taxonomy

As a plant taxonomist, most of my work involves determining how many species are in a particular genus of flowering plants, how they are related to one another, and what the correct scientific names for those species are. To do this, I gather and analyze data from a number of sources, including genetics, chromosomes, ecology, geography, and morphology. 

My colleague Leon Perrie has blogged previously about generating genetic data, including extracting DNA and analyzing microsatellite markers for Pseudopanax species, and detecting differences in DNA sequence data for maidenhair fern species.  

Here I will show how I go about collecting morphological data from herbarium specimens for taxonomic research. 

Heidi observing a Te Papa herbarium specimen of Plantago spathulata with a dissecting microscope. Photo © Museum of New Zealand Te Papa Tongarewa.

Heidi observing a Te Papa herbarium specimen of Plantago spathulata with a dissecting microscope. Photo by Carlos Lehnebach © Museum of New Zealand Te Papa Tongarewa.

The first step of deciding which characters or traits to measure and observe is key. This will vary depending on the particular plant group being studied, and can be an involved process (meaning, probably the subject of another blog!). Some examples of characters commonly used are leaf length and width, presence and type of hairs on calyx, and number of seeds per capsule. 

The next step is to measure the characters on herbarium specimens collected from throughout the geographic range of the species of interest. Because many of the traits are quite small, a 10x hand lens or (even better) a dissecting microscope is necessary to see them. 

Rulers or microscope reticles can be used for measuring, but I prefer a digital calipers. Digital calipers are very precise and can be connected to a computer so that the data is transferred directly to a spreadsheet by just pressing a button! 

Here you can see the whole set up, including Heidi measuring the specimen with the digital calipers, that are in turn connected to the laptop for instant data transfer. Photo © Museum of New Zealand Te Papa Tongarewa.

Here you can see Heidi measuring a specimen using the whole set up: dissecting microscope and digital calipers that are plugged into a laptop for instant data transfer. Photo by Carlos Lehnebach © Museum of New Zealand Te Papa Tongarewa.

Measuring leaf width (in mm) of a Te Papa specimen of Plantago spathulata with a digital calipers. Photo © Museum of New Zealand Te Papa Tongarewa.

Measuring leaf width (in mm) of a Te Papa specimen of Plantago spathulata with a digital calipers. Photo by Carlos Lehnebach © Museum of New Zealand Te Papa Tongarewa.

Laptop with spreadsheet showing highlighted cell with "13.28" mm leaf width measurement, that has been automatically transferred from the digital calipers to the morphological data matrix. Photo © Museum of New Zealand Te Papa Tongarewa.

Laptop with spreadsheet showing highlighted cell with "13.28" mm leaf width measurement, that has been automatically transferred from the digital calipers to the morphological data matrix. Photo by Heidi Meudt © Museum of New Zealand Te Papa Tongarewa.

Collecting morphological data for one plant specimen, that is, measuring and observing specific traits from the roots, leaves, flowers, and fruits, can take anywhere from 30 to 90 minutes. Multiply that by dozens or even hundreds of specimens, and you can see that collecting morphological data for taxonomic research takes time. 

Once the data matrix has been generated comes the exciting part: analysis and interpretation of the results. But I’ll leave that for another blog entry! 

Calipers and laptop with data matrix of morphological characters. Photo © Museum of New Zealand Te Papa Tongarewa.

Calipers and laptop with data matrix of morphological characters. Photo by Heidi Meudt © Museum of New Zealand Te Papa Tongarewa.

What’s it like to be a MSc student in systematic botany? Just ask Jessie…

My name is Jessie Prebble and I am the current (2009) recipient of the Te Papa MSc Scholarship in Molecular Systematics. I’m studying at Victoria University, looking at the evolution of the plant genus Wahlenbergia in New Zealand and Australia. I’m using various molecular techniques to try to determine how reliable the current taxonomy of the New Zealand species is, and whether I can infer how many times the genus invaded New Zealand, where from, and when.

Jessie and Wahlenbergia albomarginata subsp. olvina on the ultramafic Dun Mountains near Nelson, New Zealand.

Me and Wahlenbergia albomarginata subsp. olvina on the ultramafic Dun Mountains near Nelson, New Zealand. Photo © Jessie Prebble.

Here I am finding Wahlenbergia gloriosa in an alpine herbfield on Mt Kosciuszko, New South Wales, Australia. Photo © Jessie Prebble.

Here I am finding Wahlenbergia gloriosa in an alpine herbfield on Mt Kosciuszko, New South Wales, Australia. Photo © Jessie Prebble.

I love my research. I spent last summer exploring the country collecting specimens in beautiful locations from the Garvie Mountains in Southland to Muriwai Beach north of Auckland. I even got to head over to New South Wales to hunt down some of the Australian species.  I then spent a few weeks mounting and processing all of my collections, and now they’re stored in the Te Papa Herbarium.

This is the common South Island alpine plant Wahlenbergia albomarginata subsp. albomarginata, which grows profusely on the slopes of Mt Robert, Nelson Lakes area, New Zealand.

This is the common South Island alpine plant Wahlenbergia albomarginata subsp. albomarginata, which grows profusely on the slopes of Mt Robert, Nelson Lakes area, New Zealand. Photo © Jessie Prebble.

Wahlenbergia ceracea growing in an alpine bog on the slopes of Mt Kosciuszko, New South Wales, Australia.

Wahlenbergia ceracea growing in an alpine bog on the slopes of Mt Kosciuszko, New South Wales, Australia. Photo © Jessie Prebble.

Currently I’m dividing my time between the lab, where I extract and sequence short fragments of my specimens’ DNA, and the computer lab, where I puzzle my head over numerous types of data files. I have selected three regions to sequence, two from the chloroplast (trnL-F and trnK-psbA) and one nuclear ribosomal region (ITS). I explore my sequence data by forming alignments of the sequences, then creating phylogenetic trees to tease out the relationships between the species.

Results are starting to trickle in, and so far I can tell that all of the New Zealand species are very closely related, which most likely points to recent and rapid evolution here.  Further results to follow…

The beautiful coastal plant Wahlenbergia congesta subps. haastii growing on sand dunes on the South Island’s west coast, by the mouth of Ship Ck. Photo © Jessie Prebble.

The beautiful coastal plant Wahlenbergia congesta subps. haastii growing on sand dunes on the South Island’s west coast, by the mouth of Ship Ck. Photo © Jessie Prebble.

Developing the next generation of systematists

Developing “the next generation” of professionals is perhaps one of the most important duties of skilled workers in any discipline.  Te Papa’s Botany staff are involved in co-supervising postgraduate university students in systematics. We are currently calling for applications for the Te Papa MSc Scholarship in Molecular Systematics for 2010.

Te Papa is offering this scholarship in collaboration with the School of Biological Sciences at Victoria University of Wellington.  The purpose of the award is to promote research, collaboration, and mentoring between Te Papa and Victoria University in the area of molecular systematics—the scientific study of the diversity and evolutionary relationships of living organisms, based on genetic (DNA) evidence—and taxonomy (naming and classification).
 
Potential projects for 2010-2011 include investigation of species boundaries amongst Gleichenia ferns, phylogeography and/or hybridisation in Asplenium ferns, species relationships amongst Myosotis forget-me-nots, or another topic to be determined.

If you know of any keen students who may be interested, please spread the word–the deadline for applications for is 1 November 2009! 

Potential project plants: Gleichinia, Asplenium, and Myosotis.  Copyright Leon Perrie (Gleichinia and Asplenium) and Viv McGlynn (Myosotis).

Potential project plants: Gleichinia, Asplenium, and Myosotis. Images of Gleichinia and Asplenium by Leon Perrie, Curator; © Museum of New Zealand Te Papa Tongarewa. Image of Myosotis By Viv McGlynn, © Viv McGlynn.

 

Edit – 1 December 2009 – Link to the description of the scholarship removed, as it is no longer available

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